Issue 21, 2010
From the journal:

Soft Matter

Enzyme switch by complementary polymer pair system (CPPS)†

Shunsuke Tomita,a   Len Ito,bc   Hiroshi Yamaguchi,c   Gen-ichi Konishi,d   Yukio Nagasakiaefgh  and  Kentaro Shiraki*ae  

* Corresponding authors

a Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan
E-mail: shiraki@bk.tsukuba.ac.jp
Fax: + 81-29-8535215
Tel: + 81-29-8535306

b Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo, Japan

c School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo, Japan

d Department of Organic and Polymeric Materials, Graduate School of Science and Engineering, Tokyo Institute of Technology, 2-12-1-H-134 Ookayama, Meguro-ku, Tokyo, Japan

e Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki, Japan

f Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan

g Graduate School of Comprehensive Human, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan

h Satellite Laboratory of International Center for Materials Nanoarchitechtonics (MANA), National Institute of Materials Science (NIMS), 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan

Abstract

Manipulation of enzyme activity at will presents various research and industrial applications. Herein we describe development of a technology for inactivation and reactivation of enzyme activities using a polyanionic poly(acrylic acid) (PAAc) and a polycationic poly(allylamine) (PAA). Enzyme activities of ribonuclease A (RNase A), lysozyme, cellulase, and α-amylase were lost through addition of PAAc or PAA because of their binding to the enzymes. The activity of these enzymes except for α-amylase was then fully restored from the complex by the addition of oppositely charged polymers. Such manipulation of enzyme activity using a complementary polymer pair system (CPPS) enables the expansion of biomedical and biotechnological applications of the enzymes, including realization of protein delivery and intelligent bioreactors.

Graphical abstract: Enzyme switch by complementary polymer pair system (CPPS)

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C000930J
Article type
Paper
Submitted
15 Jan 2010
Accepted
13 May 2010
First published
01 Jul 2010

Soft Matter, 2010,6, 5320-5326

Permissions

Request permissions

Enzyme switch by complementary polymer pair system (CPPS)

S. Tomita, L. Ito, H. Yamaguchi, G. Konishi, Y. Nagasaki and K. Shiraki, Soft Matter, 2010, 6, 5320 DOI: 10.1039/C000930J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements