15N-Labeled ionic probes for bioanalytical mass spectrometry

Fumihiro Ito; Shin Ando; Masato Iuchi; Tomoko Nakamura; Satoko Yorita; Kentaro Yamaguchi

doi:10.1039/C003784B

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¹⁵N-Labeled ionic probes for bioanalytical mass spectrometry†

Fumihiro Ito,*^a Shin Ando,^a Masato Iuchi,^a Tomoko Nakamura,^a Satoko Yorita^a and Kentaro Yamaguchi*^a

Author affiliations

* Corresponding authors

^a Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa, Japan
E-mail: h-itoh@kph.bunri-u.ac.jp, yamaguchi@kph.bunri-u.ac.jp
Fax: +81 87 894 0181
Tel: +81 87 894 5111

Abstract

An effective La-complex-based probe ionization method is reported. Novel stable isotopically labeled probes containing the ¹⁵N-labeled 2,6-bis(oxazolin-2-yl)pyridine (pybox) ligand, succinimide-tetramethylpybox (NHS-TMpybox), maleimide-tetramethylpybox (Mal-TMpybox), and 4-(tetramethylpybox)-butyl bromoacetate (BrAc-TMpybox), have been synthesized and their value in analyzing large complex molecules has been studied. The value of the ¹⁵N-labeled pybox-La complex in ionizing various compounds, including bioactive peptides by cold-spray ionization mass spectrometry is emphasized.

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Supplementary files

¹H and ¹³C NMR spectra of 3, 8, 9, 12, 13, 14 and 15 PDF (332K)

Article information

DOI: https://doi.org/10.1039/C003784B
Article type: Paper
Submitted: 04 Mar 2010
Accepted: 25 Jun 2010
First published: 13 Aug 2010

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Org. Biomol. Chem., 2010,8, 4408-4413

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¹⁵N-Labeled ionic probes for bioanalytical mass spectrometry

F. Ito, S. Ando, M. Iuchi, T. Nakamura, Satoko Yorita and K. Yamaguchi, Org. Biomol. Chem., 2010, 8, 4408 DOI: 10.1039/C003784B

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Organic & Biomolecular Chemistry