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Issue 10, 2010
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Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor

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Abstract

We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly(N,N-dimethylacrylamide)-based support, nonselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular recognition arising in aqueous solution reactions is manifest also in the analogous templated solid phase DCL syntheses. The ability of a polymer bound template to identify and isolate a synthetic receptor via dynamic combinatorial chemistry was not compromised by the large size of the library, consisting of well over 140 theoretical members, demonstrating the practical advantages of a polymer-supported DCL methodology.

Graphical abstract: Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor

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Article information


Submitted
13 Jan 2010
Accepted
08 Mar 2010
First published
19 Mar 2010

Org. Biomol. Chem., 2010,8, 2414-2418
Article type
Paper

Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor

P. Besenius, P. A. G. Cormack, R. F. Ludlow, S. Otto and D. C. Sherrington, Org. Biomol. Chem., 2010, 8, 2414
DOI: 10.1039/C000333F

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