Issue 4, 2010

Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles

Abstract

The immobilisation of proteins on to nanoparticles has a number of applications ranging from biocatalysis through to cellular delivery of biopharmaceuticals. Here we describe a phosphopantetheinyl transferase (Sfp)-catalysed method for immobilising proteins bearing a small 12-mer “ybbR” tag on to nanoparticles functionalised with coenzyme A. The Sfp-catalysed immobilisation of proteins on to nanoparticles is a highly efficient, single step reaction that proceeds under mild conditions and results in a homogeneous population of proteins that are covalently and site-specifically attached to the surface of the nanoparticles. Several enzymes of interest for biocatalysis, including an arylmalonate decarboxylase (AMDase) and a glutamate racemase (GluR), were immobilised on to nanoparticles using this approach. These enzymes retained their activity and showed high operational stability upon immobilisation.

Graphical abstract: Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
13 Aug 2009
Accepted
06 Nov 2009
First published
23 Dec 2009

Org. Biomol. Chem., 2010,8, 782-787

Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles

L. S. Wong, K. Okrasa and J. Micklefield, Org. Biomol. Chem., 2010, 8, 782 DOI: 10.1039/B916773K

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