Jump to main content
Jump to site search

Issue 5, 2010
Previous Article Next Article

Synthesis, characterization and biocompatibility of “green” synthesized silver nanoparticles using tea polyphenols

Author affiliations

Abstract

Since ancient times, people have taken advantage of the antimicrobial effects of colloidal silver particles. Aside from the medical prospects, silver nanoparticles are found in a wide range of commercially available consumer products ranging from cosmetics to household cleansers. Current synthetic methods for creating silver nanoparticles typically call for potentially hazardous chemicals, extreme heat, and produce environmentally dangerous byproducts. Therefore, it is essential that novel “green” synthesis of nanoparticles becomes a reality, and it is imperative to fully analyze the potential toxic effects of these nanoparticles. In this study, we have shown that by reducing silver nitrate in solutions of tea extract or epicatechin of varying concentrations, spherical silver nanoparticles were formed that had controllable size distributions depending on the concentration of tea extract or epicatechin in the samples. Our ultra-resolution microscopy demonstrated that the nanoparticles were in fact interacting with the keratinocytes. Furthermore, evaluation of mitochondrial function (MTS) to assess cell viability and membrane integrity (LDH) in human keratinocytes showed that the silver nanoparticles were nontoxic. These results demonstrated that these nanoparicles are potentially biocompatible and warrant further evaluation in other biological systems.

Graphical abstract: Synthesis, characterization and biocompatibility of “green” synthesized silver nanoparticles using tea polyphenols

Back to tab navigation

Publication details

The article was received on 22 Jan 2010, accepted on 27 Jan 2010 and first published on 15 Mar 2010


Article type: Paper
DOI: 10.1039/C0NR00046A
Citation: Nanoscale, 2010,2, 763-770

  •   Request permissions

    Synthesis, characterization and biocompatibility of “green” synthesized silver nanoparticles using tea polyphenols

    M. C. Moulton, L. K. Braydich-Stolle, M. N. Nadagouda, S. Kunzelman, S. M. Hussain and R. S. Varma, Nanoscale, 2010, 2, 763
    DOI: 10.1039/C0NR00046A

Search articles by author

Spotlight

Advertisements