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Issue 5, 2010
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RNA-cleaving 10–23 deoxyribozyme with a single amino acid-like functionality operates without metal ion cofactors

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Abstract

A series of 10–23 deoxyribozymes (D2–D9) containing single amino-acid-bearing nucleosides (thr6dA, hisam6dA, hisam5dU and ncmnm5dU) at positions 4, 5, 8 or 15 of the catalytic core was obtained by chemical synthesis. The deoxyribozymes were screened for their catalytic efficiency, and in the presence of 1 mM Mg2+ two of them, containing at position 8 either hisam5dU (D8) or ncmnm5dU (D9), were found to be RNA nucleases several times more active than their non-modified precursor. Moreover, in the magnesium-free TRIS or PIPES buffers, these enzymes were able to catalyze the cleavage of the phosphodiester bond located between the 5′-GpU-3′ sequence of the complementary RNA substrate. The cleavage reaction proceeded with the highest efficiency at pH > 7.

Graphical abstract: RNA-cleaving 10–23 deoxyribozyme with a single amino acid-like functionality operates without metal ion cofactors

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Article information


Submitted
24 Nov 2009
Accepted
01 Feb 2010
First published
31 Mar 2010

New J. Chem., 2010,34, 934-948
Article type
Paper

RNA-cleaving 10–23 deoxyribozyme with a single amino acid-like functionality operates without metal ion cofactors

D. Smuga, K. Majchrzak, E. Sochacka and B. Nawrot, New J. Chem., 2010, 34, 934
DOI: 10.1039/B9NJ00705A

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