Issue 5, 2010
From the journal:

New Journal of Chemistry

Stereopure oligonucleotide phosphorothioates as human telomerase substrates†‡

Ronald Pruzan,a   Daria Zielinska,a   Beata Rebowska-Kocon,b   Barbara Nawrotb  and  Sergei M. Gryaznov*a  

* Corresponding authors

a Geron Corporation, 230 Constitution Drive, Menlo Park, USA
E-mail: Sgryaznov@geron.com

b Centre of Molecular and Macromolecular Studies of the Polish Academy of Sciences, Lodz, Poland

Abstract

To address the role of sulfur in the telomerase–primer recognition process, we investigated the effects of substitution of natural phosphodiester linked DNA telomerase substrates with phosphorothioate group-containing counterparts. Model dodecanucleotides with either stereorandom or stereopure all-Rp/Sp internucleoside phosphorothioate linkages were evaluated and the stereorandom models found to have slightly reduced affinity for the enzyme.

Graphical abstract: Stereopure oligonucleotide phosphorothioates as human telomerase substrates

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Article information

DOI
https://doi.org/10.1039/B9NJ00622B
Article type
Letter
Submitted
31 Oct 2009
Accepted
11 Dec 2009
First published
25 Jan 2010

New J. Chem., 2010,34, 870-874

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Stereopure oligonucleotide phosphorothioates as human telomerase substrates

R. Pruzan, D. Zielinska, B. Rebowska-Kocon, B. Nawrot and S. M. Gryaznov, New J. Chem., 2010, 34, 870 DOI: 10.1039/B9NJ00622B

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