Formation and study of single metal ion–phospholipid complexes in biphasic electrospray ionization mass spectrometry

Michel Prudent; Manuel A. Méndez; Daniel F. Jana; Clémence Corminboeuf; Hubert H. Girault

doi:10.1039/C003647A

Formation and study of single metal ion–phospholipid complexes in biphasic electrospray ionization mass spectrometry

Michel Prudent,^a Manuel A. Méndez,^a Daniel F. Jana,^b Clémence Corminboeuf^b and Hubert H. Girault*^a

* Corresponding authors

^a Laboratoire d’Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Station 6, CH-1015 Lausanne, Switzerland
E-mail: hubert.girault@epfl.ch

^b Laboratory for Computational Molecular Design, Ecole Polytechnique Fédérale de Lausanne, Batochime, CH-1015 Lausanne, Switzerland

Abstract

Single metal ion–phospholipid complexes are observed in biphasic electrospray ionization mass spectrometry (BESI-MS) using a dual-channel microsprayer. Such a microsprayer makes it possible to put into contact two immiscible liquids within the Taylor cone. Thus, L-α-dipalmitoyl phosphatidylcholine (DPPC) dissolved in 1,2-dichloroethane (DCE) reacts with aqueous metal cations (M = Na⁺, K⁺, Ca²⁺, Cu²⁺, La³⁺) yielding the formation of [M-DPPC_n]^z+ complexes. The number of phospholipid molecules ranges from 1 to 4 for monovalent ions, to 8 for divalent and to more than 10 for trivalent ions respectively. The large number of ligands observed involves the formation of solvent free single ion–phospholipid complexes.

Metallomics

Formation and study of single metal ion–phospholipid complexes in biphasic electrospray ionization mass spectrometry

Abstract

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