Issue 11, 2010
From the journal:

Molecular BioSystems

Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiosetransporter and a β-glucosidase in Saccharomyces cerevisiae†

Sijin Li,a   Jing Du,a   Jie Sun,ab   Jonathan M. Galazka,c   N. Louise Glass,d   Jamie H. D. Cate,c   Xiaomin Yange  and  Huimin Zhao*a  

* Corresponding authors

a Energy Biosciences Institute, Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, 600 S. Mathews Ave., Urbana IL 61801, USA
E-mail: zhao5@illinois.edu
Fax: 001 217-333-5052

b State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

c Department of Molecular and Cell Biology, University of California, Berkeley, USA

d Department of Plant and Microbial Biology, University of California, Berkeley, USA

e BP Biofuels Business Unit, University of California, 310 Calvins Hall, Berkeley, USA

Abstract

Glucose repression is one of the main limitations in mixed lignocellulosic sugar fermentation for cost-effective production of fuels and chemicals. Here we report a novel strategy to overcome glucose repression by co-expressing a cellobiose transporter and a β-glucosidase in an engineered D-xylose-utilizing Saccharomyces cerevisiae strain. The resulting strain can simultaneously utilize cellobiose and D-xylose for ethanol production.

Graphical abstract: Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C0MB00063A
Article type
Communication
Submitted
22 Jun 2010
Accepted
19 Aug 2010
First published
27 Sep 2010

Mol. BioSyst., 2010,6, 2129-2132

Permissions

Request permissions

Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae

S. Li, J. Du, J. Sun, J. M. Galazka, N. L. Glass, J. H. D. Cate, X. Yang and H. Zhao, Mol. BioSyst., 2010, 6, 2129 DOI: 10.1039/C0MB00063A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements