Issue 9, 2010
From the journal:

Molecular BioSystems

Comparative quantitation of proteome alterations induced by aging or immortalization in primary human fibroblasts and keratinocytes for clinical applications†‡

Adrian Sprenger,§abc   Victoria Küttner,§abc   Martin L. Biniossek,d   Christine Gretzmeier,ab   Melanie Boerries,ab   Claudia Mack,c   Cristina Has,c   Leena Bruckner-Tuderman*ac  and  Jörn Dengjel*ab  

* Corresponding authors

a Freiburg Institute for Advanced Studies, School of Life Science-LIFENET, University of Freiburg, Albertstr. 19, 79104 Freiburg, Germany
E-mail: bruckner-tuderman@uniklinik-freiburg.de, joern.dengjel@frias.uni-freiburg.de
Tel: +49-761-203-97208

b Center for Biological Systems Analysis, University of Freiburg, Habsburgerstr. 49, 79104 Freiburg, Germany

c Department of Dermatology, University Medical Center Freiburg, Hauptstr. 7, 79104 Freiburg, Germany

d Institute of Molecular Medicine and Cell Research, University of Freiburg, Stefan-Meier-Str. 17, 79104 Freiburg, Germany

Abstract

We monitor proteome changes of primary human skin fibroblasts and keratinocytes during cell culture and compare them to respective immortalized cell lines using stable isotope labeling by amino acids to address their suitability as cell models for clinical disease proteomics.

Graphical abstract: Comparative quantitation of proteome alterations induced by aging or immortalization in primary human fibroblasts and keratinocytes for clinical applications

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Article information

DOI
https://doi.org/10.1039/C003962D
Article type
Communication
Submitted
09 Mar 2010
Accepted
20 Apr 2010
First published
10 May 2010

Mol. BioSyst., 2010,6, 1579-1582

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Comparative quantitation of proteome alterations induced by aging or immortalization in primary human fibroblasts and keratinocytes for clinical applications

A. Sprenger, V. Küttner, M. L. Biniossek, C. Gretzmeier, M. Boerries, C. Mack, C. Has, L. Bruckner-Tuderman and J. Dengjel, Mol. BioSyst., 2010, 6, 1579 DOI: 10.1039/C003962D

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