Issue 2, 2010
From the journal:

Molecular BioSystems

Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis†‡

Yoo Seong Choi,§a   Tyler W. Johannes,§a   Michael Simurdiak,a   Zengyi Shao,a   Haige Lua  and  Huimin Zhao*a  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana IL 61801, USA
E-mail: zhao5@illinois.edu
Fax: (001) 217-333-5052

Abstract

Spectinabilin is a rare nitrophenyl-substituted polyketidemetabolite. Here we report the cloning and heterologous expression of the spectinabilin gene cluster from Streptomyces spectabilis. Unexpectedly, this gene cluster is evolutionarily closer to the aureothin gene cluster than to the spectinabilin gene cluster from Streptomyces orinoci. Moreover, the two nearly identical spectinabilin gene clusters use a distinctly different regulation mechanism.

Graphical abstract: Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis

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Article information

DOI
https://doi.org/10.1039/B923177C
Article type
Communication
Submitted
05 Nov 2009
Accepted
20 Nov 2009
First published
22 Dec 2009

Mol. BioSyst., 2010,6, 336-338

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Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis

Y. S. Choi, T. W. Johannes, M. Simurdiak, Z. Shao, H. Lu and H. Zhao, Mol. BioSyst., 2010, 6, 336 DOI: 10.1039/B923177C

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