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Issue 1, 2009
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Selective oxidative demethylation of veratric acid to vanillic acid by CYP199A4 from Rhodopseudomonas palustris HaA2

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Abstract

CYP199A4 (RPB3613) from Rhodopseudomonas palustris HaA2 is a hememonooxygenase that catalyzes the hydroxylation of para-substituted benzoic acids. Monooxygenase activity of CYP199A4 can be reconstituted in a Class I electron transfer chain with an associated [2Fe–2S] ferredoxin, HaPux, (RPB3614) and the flavin-dependent reductase, HaPuR, (RPB3656) that is not associated with a CYPgene. CYP199A4 and the ferredoxin HaPux are produced in greater quantities using recombinant Escherichia coli expression systems when compared to the equivalent proteins in the closely related CYP199A2–Pux–PuR Class I system from R. palustris CGA009. HaPuR and HaPux can also replace PuR and Pux in supporting the CYP199A2 enzyme turnover with high activity. Whole-cell in vivo substrate oxidation systems for CYP199A4 and CYP199A2 with HaPux and HaPuR as the electron transfer proteins have been constructed. These E. coli systems were capable of selectively demethylating veratric acid at the para position to produce vanillic acid at rates of up to 15.3 μM (g-cdw)−1 min−1 and yields of up to 1.2 g L−1.

Graphical abstract: Selective oxidative demethylation of veratric acid to vanillic acid by CYP199A4 from Rhodopseudomonas palustris HaA2

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Publication details

The article was received on 07 Jul 2009, accepted on 13 Aug 2009 and first published on 14 Sep 2009


Article type: Paper
DOI: 10.1039/B913487E
Citation: Mol. BioSyst., 2009,6, 206-214

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    Selective oxidative demethylation of veratric acid to vanillic acid by CYP199A4 from Rhodopseudomonas palustris HaA2

    S. G. Bell, A. B. H. Tan, E. O. D. Johnson and L. Wong, Mol. BioSyst., 2009, 6, 206
    DOI: 10.1039/B913487E

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