Jump to main content
Jump to site search

Issue 21, 2010
Previous Article Next Article

Large-scale arrays of picolitre chambers for single-cell analysis of large cell populations

Author affiliations

Abstract

We present a new method to analyze the cytoplasmic contents of single cells in large cell populations. This new method consists of an array of microchambers in which individual cells are collected, enclosed, and lysed to create a reaction mixture of the cytoplasm with extracellular detection agents. This approach was tested for the analysis of red blood cells in 10 000 microchambers in parallel. Single cells were routinely collected in more than 60% of microchambers, the collected cells were robustly (up to 99%) lysed by electric fields, and the cytoplasm enclosed in each microchamber was analyzed with fluorescence microscopy. Using a heterogeneous cell mixture, we verified that the new method could distinguish individual cells by cytoplasmic composition and the analysis compared well with conventional flow-cytometric evaluation of mixed cell populations. In contrast to flow-cytometry, the new method monitored single cells over time, thus characterizing the distributions of caspase activities of 5000 individual cells. This approach should be interesting for a variety of applications that would benefit from the ability to measure the distribution of cytoplasmic compounds in complex cell populations, including hematology, oncology, and immunology.

Graphical abstract: Large-scale arrays of picolitre chambers for single-cell analysis of large cell populations

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 Jun 2010, accepted on 06 Aug 2010 and first published on 13 Sep 2010


Article type: Paper
DOI: 10.1039/C0LC00139B
Lab Chip, 2010,10, 2952-2958

  •   Request permissions

    Large-scale arrays of picolitre chambers for single-cell analysis of large cell populations

    W. C. Lee, S. Rigante, A. P. Pisano and F. A. Kuypers, Lab Chip, 2010, 10, 2952
    DOI: 10.1039/C0LC00139B

Search articles by author

Spotlight

Advertisements