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Issue 22, 2010
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Size-selective immunofluorescence of Mycobacterium tuberculosiscells by capillary- and viscous forces

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Abstract

Rapid, low cost screening of tuberculosis requires an effective enrichment method of Mycobacterium tuberculosis (MTB) cells. Currently, microfiltration and centrifugation steps are frequently used for sample preparation, which are cumbersome and time-consuming. In this study, the size-selective capturing mechanism of a microtip-sensor is presented to directly enrich MTB cells from a sample mixture. When a microtip is withdrawn from a spherical suspension in the radial direction, the cells that are concentrated by AC electroosmosis are selectively enriched to the tip due to capillary- and viscous forces. The size-selectivity is characterized by using polystyrene microspheres, which is then applied to size-selective capture of MTB from a sample mixture. Our approach yields a detection limit of 800 cells mL−1, one of the highest-sensitivity immunosensors to date.

Graphical abstract: Size-selective immunofluorescence of Mycobacterium tuberculosis cells by capillary- and viscous forces

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Supplementary files

Article information


Submitted
08 Jun 2010
Accepted
06 Aug 2010
First published
23 Sep 2010

Lab Chip, 2010,10, 3178-3181
Article type
Technical Note

Size-selective immunofluorescence of Mycobacterium tuberculosis cells by capillary- and viscous forces

W. Yeo, F. Chou, G. Fotouhi, K. Oh, B. T. Stevens, H. Tseng, D. Gao, A. Q. Shen, J. Chung and K. Lee, Lab Chip, 2010, 10, 3178
DOI: 10.1039/C0LC00077A

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