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Issue 9, 2010
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Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

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Abstract

Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL−1 of bFGF on 20 μg mL−1 of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.

Graphical abstract: Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

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Supplementary files

Article information


Submitted
04 Nov 2009
Accepted
11 Feb 2010
First published
16 Mar 2010

Lab Chip, 2010,10, 1113-1119
Article type
Communication

Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

K. Kamei, M. Ohashi, E. Gschweng, Q. Ho, J. Suh, J. Tang, Z. T. For Yu, A. T. Clark, A. D. Pyle, M. A. Teitell, Ki-Bum. Lee, O. N. Witte and H. Tseng, Lab Chip, 2010, 10, 1113
DOI: 10.1039/B922884E

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