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Issue 4, 2010
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Microfluidic isolation and transcriptome analysis of serum microvesicles

Chihchen Chen,a  Johan Skog,b  Chia-Hsien Hsu,a  Ryan T. Lessard,b  Leonora Balaj,b  Thomas Wurdinger,bc  Bob S. Carter,d  Xandra O. Breakefield,b  Mehmet Tonera  and  Daniel Irimia*a 
Author affiliations

* Corresponding authors

a BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Shriners Hospital for Children, Harvard Medical School, Boston, USA
E-mail: dirimia@hms.harvard.edu
Fax: +1 617 724 2999
Tel: +1 617 371 4883

b Departments of Neurology and Radiology, Massachusetts General Hospital, Neuroscience Program, Harvard Medical School, Boston, USA

c Neuro-oncology Research Group, Department of Neurosurgery, VU University Medical Center, Amsterdam, the Netherlands

d Departments of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, USA

Abstract

Microvesicles (exosomes) shed from both normal and cancerous cells may serve as means of intercellular communication. These microvesicles carry proteins, lipids and nucleic acids derived from the host cell. Their isolation and analysis from blood samples have the potential to provide information about state and progression of malignancy and should prove of great clinical importance as biomarkers for a variety of disease states. However, current protocols for isolation of microvesicles from blood require high-speed centrifugation and filtration, which are cumbersome and time consuming. In order to take full advantage of the potential of microvesicles as biomarkers for clinical applications, faster and simpler methods of isolation will be needed. In this paper, we present an easy and rapid microfluidic immunoaffinity method to isolate microvesicles from small volumes of both serum from blood samples and conditioned medium from cells in culture. RNA of high quality can be extracted from these microvesicles providing a source of information about the genetic status of tumors to serve as biomarkers for diagnosis and prognosis of cancer.

Graphical abstract: Microfluidic isolation and transcriptome analysis of serum microvesicles

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Publication details

The article was received on 06 Aug 2009, accepted on 05 Nov 2009 and first published on 08 Dec 2009


Article type: Paper
DOI: 10.1039/B916199F
Citation: Lab Chip, 2010,10, 505-511

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    Microfluidic isolation and transcriptome analysis of serum microvesicles

    C. Chen, J. Skog, C. Hsu, R. T. Lessard, L. Balaj, T. Wurdinger, B. S. Carter, X. O. Breakefield, M. Toner and D. Irimia, Lab Chip, 2010, 10, 505
    DOI: 10.1039/B916199F

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