Issue 6, 2010
From the journal:

Journal of Materials Chemistry

Calcium phosphate/PLGA-mPEG hybrid porous nanospheres: A promising vector with ultrahigh gene loading and transfection efficiency

Ke-Wei Wang,a   Lin-Zhu Zhou,b   Ying Sun,c   Guo-Jun Wu,b   Hong-Chen Gu,*b   You-Rong Duan,*c   Feng Chena  and  Ying-Jie Zhu*a  

* Corresponding authors

a State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, P. R. China
E-mail: y.j.zhu@mail.sic.ac.cn

b Med-X Institute, Shanghai Jiao Tong University, Shanghai, P. R. China
E-mail: hcgu@sjtu.edu.cn

c Cancer Institute of Shanghai Jiao Tong University, Shanghai, P. R. China
E-mail: yrduan@shsci.org

Abstract

A facile room-temperature method for the fabrication of calcium phosphate (CaP)/PLGA-mPEG hybrid porous nanospheres has been developed. The hybrid CaP/PLGA-mPEG nanospheres obtained were 20∼60 nm in diameter and exhibited porous structure, and they were applied as DNA vectors for DNA loading and in vitro transfection. The results showed that the DNA binding capacity and transfection efficiency of the as-prepared hybrid porous nanospheres were much higher than those of the CaP precipitates prepared according to the standard CaP transfection procedure and mesoporous silica reported before. MTT assays confirmed that the CaP/PLGA-mPEG hybrid porous nanospheres were quite safe. In addition, both CaP and PLGA-mPEG are biocompatible and biodegradable, thus the as-prepared CaP/PLGA-mPEG hybrid porous nanospheres are promising in gene delivery.

Graphical abstract: Calcium phosphate/PLGA-mPEG hybrid porous nanospheres: A promising vector with ultrahigh gene loading and transfection efficiency

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Article information

DOI
https://doi.org/10.1039/B917441A
Article type
Paper
Submitted
24 Aug 2009
Accepted
12 Nov 2009
First published
15 Dec 2009

J. Mater. Chem., 2010,20, 1161-1166

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Calcium phosphate/PLGA-mPEG hybrid porous nanospheres: A promising vector with ultrahigh gene loading and transfection efficiency

K. Wang, L. Zhou, Y. Sun, G. Wu, H. Gu, Y. Duan, F. Chen and Y. Zhu, J. Mater. Chem., 2010, 20, 1161 DOI: 10.1039/B917441A

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