Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 1, 2010
Previous Article Next Article

In vitro biocompatibility of nanoscale zerovalent iron particles (NZVI) synthesized using tea polyphenols

Author affiliations

Abstract

A “green” protocol was used for the rapid generation of nanoscale zerovalent iron (NZVI) particles using tea polyphenols. The NZVI particles were subsequently examined for in vitro biocompatibility using the human keratinocyte cell (HaCaT) line as a representative skin exposure model. The cells were exposed to NZVI for time periods of 24 and 48 h. Biocompatibility was assessed using the methyl tetrazolium, or MTS, (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)) and lactate dehydrogenase (LDH) assays to determine in vitro cytotoxicity. The evaluation of mitochondrial function (MTS) and membrane integrity (LDH) in human keratinocytes showed that these “green” synthesized NZVI particles were nontoxic in the human keratinocytes exposed when compared with control samples synthesized using a borohydride protocol. In fact, in most cases, these “green” nanoparticles induced a prolific response in the cellular function even at the highest concentration (100μg ml−1).

Graphical abstract: In vitro biocompatibility of nanoscale zerovalent iron particles (NZVI) synthesized using tea polyphenols

Back to tab navigation

Publication details

The article was received on 07 Apr 2009, accepted on 09 Oct 2009 and first published on 04 Nov 2009


Article type: Paper
DOI: 10.1039/B921203P
Citation: Green Chem., 2010,12, 114-122

  •   Request permissions

    In vitro biocompatibility of nanoscale zerovalent iron particles (NZVI) synthesized using tea polyphenols

    M. N. Nadagouda, A. B. Castle, R. C. Murdock, S. M. Hussain and R. S. Varma, Green Chem., 2010, 12, 114
    DOI: 10.1039/B921203P

Search articles by author

Spotlight

Advertisements