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Issue 12, 2010
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Impact of microcystin containing diets on physiological performance of Nile tilapia (Oreochromis niloticus) concerning detoxification

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Abstract

Nile tilapia (Oreochromis niloticus) were fed by diets supplemented with cyanobacteria containing in part the cyanotoxin microcystin-LR (MC-LR) to determine the potential impacts on detoxification. Four different diets were prepared based on a commercial diet: (1) control, (2) MC-5% (containing 5% dried Microcystis sp. biomass with 4.92 μg MC-LR g−1 diet), (3) MC-20% (containing 20% dried Microcystis sp. biomass with 19.54 μg MC-LR g−1 diet), and (4) Arthr-20% (containing 20% dried Arthrospira sp. biomass without MC-LR). Blood and liver samples were taken after one, 7, and 28 days and protein has been determined in plasma and liver. In the liver, impacts on detoxification were measured by glutathione-S-transferase (GST) activities and gene expression of multi drug resistance protein (MDRP). Plasma protein did not change between all four diets at any sampling time whereas liver protein was significantly elevated already after one day in Arthr-20% and after 28 days in both, MC-20% and Arthr-20%. Biochemical measurements of GST activities revealed no significant impact at any sampling time. In order to characterize the potential effect of MC-LR on MDRP, RT-qPCR method was established. However, as for GST activities no significant changes in MDRP gene expression have been observed. Thus, in summary, oral exposure of MC-LR containing cyanobacteria to Nile tilapia via feed ingestion did not impact significantly detoxification in liver concerning GST activities and MDRP expression despite biochemical composition concerning liver protein was significantly elevated by the diets containing 20% cyanobacteria biomass, regardless whether they contained MC-LR or not.

Graphical abstract: Impact of microcystin containing diets on physiological performance of Nile tilapia (Oreochromis niloticus) concerning detoxification

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Publication details

The article was received on 25 May 2010, accepted on 02 Sep 2010 and first published on 15 Oct 2010


Article type: Paper
DOI: 10.1039/C0EM00246A
Citation: J. Environ. Monit., 2010,12, 2276-2281
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    Impact of microcystin containing diets on physiological performance of Nile tilapia (Oreochromis niloticus) concerning detoxification

    A. Ziková, A. Trubiroha, C. Wiegand, S. Wuertz, B. Rennert, S. Pflugmacher, R. Kopp, J. Mareš, P. Spurný and W. Kloas, J. Environ. Monit., 2010, 12, 2276
    DOI: 10.1039/C0EM00246A

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