Issue 39, 2010
From the journal:

Physical Chemistry Chemical Physics

Enzyme immobilisation on self-organised nanopatterned electrode surfaces

Janine Gajdzik,a   Jennifer Lenz,a   Harald Natter,a   Rolf Hempelmann,*a   Gert-Wieland Kohring,b   Friedrich Giffhorn,b   Mila Manolovac  and  Dieter M. Kolbc  

* Corresponding authors

a Physical Chemistry, Saarland University, 66123 Saarbrücken, Germany
E-mail: r.hempelmann@mx.uni-saarland.de
Fax: +49 681-302-4759
Tel: +49 681-302-4750

b Applied Microbiology, Saarland University, 66123 Saarbrücken, Germany
E-mail: gkohring@mx.uni-saarland.de
Fax: +49 681-302-4360
Tel: +49 681-302-3657

c Institute of Electrochemistry, Ulm University, 89069 Ulm, Germany
E-mail: dieter.kolb@uni-ulm.de
Fax: +49 731-502-5409
Tel: +49 731-502-5400

Abstract

A new method is described for immobilisation of enzymes on polymer-coated Pt islands. These islands are deposited on top of a SAM-covered Au(111) electrode by a combination of electroless and electrochemical deposition, which allows for a variation of island size and distance between the islands. Here we describe the immobilisation of pyranose-2-oxidase (P2Ox) and the catalytic response to D-glucose on such a nanopatterned surface, which provides optimum access to the active centres of the enzyme.

Graphical abstract: Enzyme immobilisation on self-organised nanopatterned electrode surfaces

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C0CP00893A
Article type
Paper
Submitted
16 Jun 2010
Accepted
12 Aug 2010
First published
10 Sep 2010

Phys. Chem. Chem. Phys., 2010,12, 12604-12607

Permissions

Request permissions

Enzyme immobilisation on self-organised nanopatterned electrode surfaces

J. Gajdzik, J. Lenz, H. Natter, R. Hempelmann, G. Kohring, F. Giffhorn, M. Manolova and D. M. Kolb, Phys. Chem. Chem. Phys., 2010, 12, 12604 DOI: 10.1039/C0CP00893A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements