Issue 46, 2010
From the journal:

Chemical Communications

Cleavage specificities of the brother and sister proteases Lys-C and Lys-N†

Reinout Raijmakers,ab   Pieter Neerincx,ab   Shabaz Mohammedab  and  Albert J. R. Heck*abc  

* Corresponding authors

a Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
E-mail: a.j.r.heck@uu.nl
Tel: 0031 302536797

b Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, the Netherlands

c Centre for Biomedical Genetics, Padualaan 8, 3584 CH Utrecht, the Netherlands

Abstract

Understanding of protease specificity is essential in proteomics strategies to correctly compare peptide fragmentation spectra with spectra predicted from data extracted from sequence databases. Here we evaluate the specificities of two proteolytic enzymes Lys-C and Lys-N, which cleave peptide-bonds at, respectively, the C- and N-terminal side of a lysine residue.

Graphical abstract: Cleavage specificities of the brother and sister proteases Lys-C and Lys-N

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Article information

DOI
https://doi.org/10.1039/C0CC02523B
Article type
Communication
Submitted
12 Jul 2010
Accepted
22 Sep 2010
First published
18 Oct 2010

Chem. Commun., 2010,46, 8827-8829

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Cleavage specificities of the brother and sister proteases Lys-C and Lys-N

R. Raijmakers, P. Neerincx, S. Mohammed and A. J. R. Heck, Chem. Commun., 2010, 46, 8827 DOI: 10.1039/C0CC02523B

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