Issue 39, 2010
From the journal:

Chemical Communications

Diazirine-containing RNA photocrosslinking probes for the study of siRNA–protein interactions†

Satoru Kuboe,‡a   Mayuko Yoda,‡cd   Aya Ogata,a   Yukio Kitade,ab   Yukihide Tomari*cd  and  Yoshihito Ueno*ab  

* Corresponding authors

a Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
E-mail: uenoy@gifu-u.ac.jp
Fax: +81-58-293-2794
Tel: +81-58-293-2639

b United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Japan

c Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan
E-mail: tomari@iam.u-tokyo.ac.jp
Fax: +81-3-5841-8485
Tel: +81-3-5841-7839

d Department of Medical Genome Sciences, The University of Tokyo, Japan

Abstract

We here report the synthesis and characterization of small interfering RNAs with aryl trifluoromethyl diazirine moieties in the 3′-overhang regions, which allow sensitive detection of interacting proteins during assembly of the effector ribonucleoprotein complex by irradiation with minimally destructive long-wavelength ultraviolet light.

Graphical abstract: Diazirine-containing RNA photocrosslinking probes for the study of siRNA–protein interactions

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Article information

DOI
https://doi.org/10.1039/C0CC02450C
Article type
Communication
Submitted
09 Jul 2010
Accepted
16 Aug 2010
First published
06 Sep 2010

Chem. Commun., 2010,46, 7367-7369

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Diazirine-containing RNA photocrosslinking probes for the study of siRNA–protein interactions

S. Kuboe, M. Yoda, A. Ogata, Y. Kitade, Y. Tomari and Y. Ueno, Chem. Commun., 2010, 46, 7367 DOI: 10.1039/C0CC02450C

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