Issue 13, 2010

Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples

Abstract

We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.

Graphical abstract: Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples

Supplementary files

Article information

Article type
Communication
Submitted
02 Dec 2009
Accepted
08 Feb 2010
First published
25 Feb 2010

Chem. Commun., 2010,46, 2212-2214

Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples

P. L. Urban, A. Amantonico, S. R. Fagerer, P. Gehrig and R. Zenobi, Chem. Commun., 2010, 46, 2212 DOI: 10.1039/B925433A

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