Issue 12, 2010

Detection of influenza viruses by a waveguide-mode sensor

Abstract

Methods are demonstrated for antibody-based detection of influenza viruses using the monolithic-sensing-plate technology of an evanescent-field-coupled waveguide-mode sensor. To facilitate the detection of viruses at lower concentrations, a strategy of complexing the virus with Coomassie Brilliant Blue dye was used to amplify the signal. By applying this method, hemagglutinins from avian influenza virus (H5N1) and human influenza virus (A/Panama/2007/1999) were detected. An intact human influenza virus (A/Brisbane/59/2007) was also detected. The method is suitable for the detection of virulent viruses that may emerge at various stages of evolution.

Graphical abstract: Detection of influenza viruses by a waveguide-mode sensor

Supplementary files

Article information

Article type
Paper
Submitted
10 Aug 2010
Accepted
22 Sep 2010
First published
26 Oct 2010

Anal. Methods, 2010,2, 1880-1884

Detection of influenza viruses by a waveguide-mode sensor

S. C. B. Gopinath, K. Awazu and M. Fujimaki, Anal. Methods, 2010, 2, 1880 DOI: 10.1039/C0AY00491J

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