High selectivity chemiluminescence sensor for determination of puerarin in diet foods/weight loss promoters based on novel rhodanine and monodisperse molecularly imprinted microspheres

Abstract

Puerarin-monodisperse molecularly imprinted microspheres were prepared by suspension polymerization. The binding property was investigated by calculating imprinting-induced binding promotion. Using monodisperse microspheres as the recognition material, rhodanine as the chemiluminescence probe, a novel chemiluminescence sensor was developed for the determination of puerarin in diet foods/weight loss promoters. The flow path was designed to obtain a continuous chemiluminescence signal. Due to the combination of special morphology of the monodisperse microspheres and excellent properties of the novel chemiluminescence system, this sensor displayed good selectivity and sensitivity. Under optimum conditions, the CL intensity has a linear relationship versus the concentration of puerarin, the linear range is 1 × 10⁻⁷ – 3 × 10⁻⁵ g mL⁻¹ with a low detection limit of 3.4 × 10⁻⁸ g mL⁻¹. The proposed method showed satisfactory application in the determination of puerarin in diet foods/weight loss promoters.