Issue 8, 2010

High-throughput surface plasmon resonance imaging-based biomolecular kinetic screening analysis

Abstract

In this paper we show a high-throughput method to screen the kinetics and affinity constants of many biomolecular interactions simultaneously. During the preparation of the sensor chip, ligands were serially diluted and spotted in a 6 × 4 microarray on the sensor surface. A multi-analyte sample was injected and the real-time surface plasmon resonance (SPR) responses of all the 24 microarray spots were obtained using a commercial SPR imaging instrument. The multi responses of the association and dissociation processes obtained from a single analyte injection are sufficient to calculate the rates and affinity constants of the interactions between three interactant antigen-antibody pairs using a simple monophasic kinetic model. The method drastically reduces the measurement time and cost in the benefit of increased throughput.

Graphical abstract: High-throughput surface plasmon resonance imaging-based biomolecular kinetic screening analysis

Supplementary files

Article information

Article type
Paper
Submitted
16 Feb 2010
Accepted
27 Apr 2010
First published
28 May 2010

Anal. Methods, 2010,2, 1020-1025

High-throughput surface plasmon resonance imaging-based biomolecular kinetic screening analysis

G. Krishnamoorthy, J. Bianca Beusink and R. B. M. Schasfoort, Anal. Methods, 2010, 2, 1020 DOI: 10.1039/C0AY00112K

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