Jump to main content
Jump to site search

Issue 7, 2010
Previous Article Next Article

Comparison and improvement of the existing methods for the determination of aflatoxins in human serum by LC-MS/MS

Author affiliations

Abstract

Aflatoxins are toxic metabolites of some Aspergillus flavus, A. parasiticus and A. nomius strains that occur in many foods and feeds. Aflatoxin B1 is the most abundant and toxic member of the family, and it is also the most potent hepatocarcinogen known. In order to estimate the potential human health risk of aflatoxin B1, it is useful to measure its blood concentration. In this work a rapid liquid chromatography coupled to mass spectrometry (LC-MS/MS) method for the simultaneous determination of aflatoxins B1, B2, G1 and G2 and its metabolites, aflatoxins M1 and M2 in human serum, is proposed. Small serum volumes have been used and the extracts have been obtained without any clean-up procedure. The isolation of the aflatoxins from human serum was achieved using subsequent pH-controlled solvent extractions steps. Recovery rates in the range between 31 and 98% were achieved in the serum, while the limits of detection and quantification for the proposed method were 0.5 ng mL−1 and 1.0 ng mL−1, respectively. The validation of the method has been demonstrated by analysing spiked and naturally contaminated blood serum.

Graphical abstract: Comparison and improvement of the existing methods for the determination of aflatoxins in human serum by LC-MS/MS

Back to tab navigation

Publication details

The article was received on 18 Dec 2009, accepted on 14 Apr 2010 and first published on 20 May 2010


Article type: Paper
DOI: 10.1039/B9AY00316A
Anal. Methods, 2010,2, 884-889

  •   Request permissions

    Comparison and improvement of the existing methods for the determination of aflatoxins in human serum by LC-MS/MS

    A. Santini, R. Ferracane, G. Meca and A. Ritieni, Anal. Methods, 2010, 2, 884
    DOI: 10.1039/B9AY00316A

Search articles by author

Spotlight

Advertisements