Issue 6, 2010

Volatile compounds from leaf extracts of Juniperus excelsa growing in Syria viagas chromatography mass spectrometry

Abstract

This study reports the use of gas chromatography mass spectrometry (GC-MS) to investigate volatile compounds in leaves of Juniperus excelsa native to Syria. Leaf samples were collected from both 100-year and 10-year old J. excelsa plants. Dried leaf samples were extracted with hexane to obtain essential oil metabolites and other non-polar compounds. GC-MS was used to profile and analyze metabolites in hexane extracts. Mass spectral deconvolution and identification and analysis of KI values allowed us to characterize sixty-nine metabolites, including twenty-four monoterpenes; twenty-nine sesquiterpenes; and sixteen other compounds including alkanes. Among these sixty-nine metabolites, germacrene B, cedrol, γ-elemene, and stenol were produced in leaf extracts of both 100-year and 10-year old trees. Interestingly, we observed that ten monoterpene and nineteen sesquiterpene compounds produced in leaves of the 100-year old trees were not detected in ones of the 10-year old trees investigated. In contrast, junipene was a dominant essential oil component in leaves of the 10-year old trees, but was either just at a detectable level or undetectable in leaves of the 100-year old trees.

Graphical abstract: Volatile compounds from leaf extracts of Juniperus excelsa growing in Syria via gas chromatography mass spectrometry

Supplementary files

Article information

Article type
Paper
Submitted
06 Sep 2009
Accepted
15 Mar 2010
First published
12 Apr 2010

Anal. Methods, 2010,2, 673-677

Volatile compounds from leaf extracts of Juniperus excelsa growing in Syria via gas chromatography mass spectrometry

K. Almaarri, L. Alamir, Y. Junaid and D. Xie, Anal. Methods, 2010, 2, 673 DOI: 10.1039/B9AY00256A

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