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Issue 7, 2010
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A direct continuous fluorometric turn-on assay for monoamine oxidase B and its inhibitor-screening based on the abnormal fluorescent behavior of silole

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Abstract

As essential enzymes in the body, monoamine oxidases (MAOs), including MAO-B, help maintain the proper balance of neurotransmitters and other dietary and biogenic amines. Thus, development of convenient and continuous assays for MAO-B and its inhibitor-screening is highly desirable since the inhibitors have implications for therapeutics for many brain diseases. In this paper, we report a direct continuous fluorescence turn-on assay for MAO-B and its inhibitor-screening with the ensemble of silole 1, heptylamine and HSO3, which are either easily accessible or commercially available. Fluorescent spectral investigations show that this fluorometric assay can be performed continuously in aqueous solution and MAO-B of concentrations as low as 0.25 μg/mL can be analyzed. Moreover, the ensemble of silole 1, heptylamine, HSO3 and MAO-B can be employed to screen the inhibitors of MAO-B.

Graphical abstract: A direct continuous fluorometric turn-on assay for monoamine oxidase B and its inhibitor-screening based on the abnormal fluorescent behavior of silole

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Publication details

The article was received on 23 Mar 2010, accepted on 02 May 2010 and first published on 27 May 2010


Article type: Paper
DOI: 10.1039/C0AN00168F
Analyst, 2010,135, 1779-1784

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    A direct continuous fluorometric turn-on assay for monoamine oxidase B and its inhibitor-screening based on the abnormal fluorescent behavior of silole

    L. Peng, G. Zhang, D. Zhang, Y. Wang and D. Zhu, Analyst, 2010, 135, 1779
    DOI: 10.1039/C0AN00168F

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