Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 2, 2010
Previous Article Next Article

Detection ofnitric oxide in tissue samples by ESI-MS

Author affiliations

Abstract

A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chosen as a probe for the detection of NO by mass spectrometry (MS) in biological tissue samples. The o-phenylenediamine group reacts with NO to form a characteristic benzotriazole. The product was identified using electrospray ionization mass spectrometry (ESI-MS) and the method validated within the range of 95–1900 nM. NO levels associated with tissue biopsies (∼10 mg) from rat vasculature and intestine tissue biopsies have been successfully determined. The different rates of NO generated from tissue samples under hypoxic and normoxic conditions have been studied by this simple and sensitive method.

Graphical abstract: Detection of nitric oxide in tissue samples by ESI-MS

Back to tab navigation

Article information


Submitted
27 Aug 2009
Accepted
24 Nov 2009
First published
30 Nov 2009

Analyst, 2010,135, 302-305
Article type
Paper

Detection of nitric oxide in tissue samples by ESI-MS

Z. Shen, A. Webster, K. J. Welham, C. E. Dyer, J. Greenman and S. J. Haswell, Analyst, 2010, 135, 302
DOI: 10.1039/B917643H

Social activity

Search articles by author

Spotlight

Advertisements