Multiplex tumor marker detection with new chemiluminescent immunoassay based on silica colloidal crystal beads

Abstract

A new multiplex chemiluminescent immunoassay (CLIA) based on silica colloidal crystal beads (SCCBs) was developed for tumor marker detection. As the code is the characteristic reflection peak originating from the stop-band of colloid crystal, they avoid photobleaching, the potential interference of encoding fluorescence with analyte-detection fluorescence and chemical instability. Meanwhile our SCCBs suspension array improved the luminescence analysis efficiency by using chemiluminescent detection of enzyme labels. By forming a sandwich immunocomplex on SCCBs, the proposed suspension array was used for simultaneous multiplex detection of tumor markers in one test tube. The results showed that the linear range was 0.5–100ng ml⁻¹ and 1.0–120ng ml⁻¹ for carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) with a detection limit of 0.12ng ml⁻¹ and 0.16ng ml⁻¹ at 3σ. The proposed array showed the storage stability and the accuracy for sample detection were acceptable, and the results were in acceptable agreement with the reference electrochemiluminescence method. This technique provided an automated, simple, sensitive and low-cost approach for multianalyte immunoassay.