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Issue 21, 2009
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Smart surfaces for pH controlled cell staining

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In this work, novel smart surfaces for in situcell staining were realized by covalent attachment of pH-responsive microgels on platforms dedicated to microfluidics for lab-on-chip. The poly(methacrylic acid) microgels were firstly synthesized in solution and then covalently immobilized on a glass surface. As they preserve their pH-sensitive nature after the covalent immobilization, microgels were loaded with an oligothiophene-conjugated anti-human CD4 monoclonal antibody, and finally incubated with a Jurkat T-cell suspension. The physiological pH of the extracellular environment induced the pH-triggered release of the labeled antiCD4 antibodies and the selective staining of the CD4-positive subpopulations within the Jurkat cell suspension. The realization of this type of smart surface for the encapsulation of specific monoclonal antibodies and their release in an on-demand way should have an enormous potential in developing fully integrated platforms for cell analysis.

Graphical abstract: Smart surfaces for pH controlled cell staining

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The article was received on 16 Jul 2009, accepted on 08 Sep 2009 and first published on 29 Sep 2009

Article type: Communication
DOI: 10.1039/B914277K
Citation: Soft Matter, 2009,5, 4101-4103
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    Smart surfaces for pH controlled cell staining

    S. Argentiere, L. Blasi, G. Ciccarella, A. Cazzato, G. Barbarella, R. Cingolani and G. Gigli, Soft Matter, 2009, 5, 4101
    DOI: 10.1039/B914277K

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