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Issue 8, 2009
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Cryophotolysis of a caged oxygen compound for use in low temperature biological studies

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Abstract

Mechanistic investigations of biological enzymatic processes require controlled initiation and monitoring of catalytic reactions. A well-known technique to trap and observe reaction intermediates building up along a reaction pathway is the use of low temperature conditions. Here, we report a kinetically competent system for the release of molecular oxygen at cryogenic temperature, using a cobalt-based caged oxygen molecule, (μ-peroxo)(μ-hydroxo)bis[bis(bipyridyl)cobalt(III)] nitrate. Cryophotolysis of this compound was induced using 266 nm laser light and monitored by absorption microspectrophotometry. Furthermore, to verify that photo-fragmentation was accompanied by release of the active caged molecule, the production of dioxygen during cryophotolysis was directly visualized. This work lays the foundations for the use of low temperature reaction triggering as a tool to prolong the lifetime of normally unstable intermediate states in oxygen-dependant enzymes.

Graphical abstract: Cryophotolysis of a caged oxygen compound for use in low temperature biological studies

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Publication details

The article was received on 02 Dec 2008, accepted on 01 May 2009 and first published on 01 Jun 2009


Article type: Paper
DOI: 10.1039/B821516B
Photochem. Photobiol. Sci., 2009,8, 1150-1156

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    Cryophotolysis of a caged oxygen compound for use in low temperature biological studies

    A. R. Howard-Jones, V. Adam, A. Cowley, J. E. Baldwin and D. Bourgeois, Photochem. Photobiol. Sci., 2009, 8, 1150
    DOI: 10.1039/B821516B

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