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Issue 24, 2009
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Assessing the cluster glycoside effect during the binding of concanavalin A to mannosylated artificial lipid rafts

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Abstract

Mannosyl glycolipids with perfluoroalkyl membrane anchors have been synthesised. When inserted into vesicles, these mannosyl lipids either dispersed evenly over the surface or, in the presence of cholesterol, phase-separated into artificial lipid rafts. At 1% mol/mol, the affinity of dispersed mannosyl lipids for Con A was 3-fold weaker than in solution, perhaps reflecting steric blocking by the surface. However increasing membrane loading 5-fold increased Con A affinity by up to 75% and indicated weak intramembrane chelation of Con A. Despite this observation, concentrating the mannosyl lipids into artificial lipid rafts did not significantly improve affinity for Con A. This lack of a cluster glycoside effect was ascribed to lipid congestion inhibiting intra-raft chelation of Con A, and implies that glycolipids located in lipid rafts may not necessarily be preorganised for multivalent binding.

Graphical abstract: Assessing the cluster glycoside effect during the binding of concanavalin A to mannosylated artificial lipid rafts

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Publication details

The article was received on 04 Jun 2009, accepted on 23 Sep 2009 and first published on 26 Oct 2009


Article type: Paper
DOI: 10.1039/B910976E
Citation: Org. Biomol. Chem., 2009,7, 5245-5254
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    Assessing the cluster glycoside effect during the binding of concanavalin A to mannosylated artificial lipid rafts

    G. T. Noble, S. L. Flitsch, K. P. Liem and S. J. Webb, Org. Biomol. Chem., 2009, 7, 5245
    DOI: 10.1039/B910976E

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