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Issue 9, 2009
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Evaluation of fluorescent polysaccharide nanoparticles for pH-sensing

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Abstract

The comprehensive characterization of novel dextran nanoparticles with regard to their suitability as pH-sensors for analytical applications (e.g. in physiology) is described. The nanoparticles are labeled with both a pH-indicator dye (fluorescein isothiocyanate, FITC) and a reference dye (sulforhodamine B acid chloride) as an internal standard. The fluorescence intensity of FITC increases with increasing pH, whereas the signal of the reference dye remains constant. Plotting the ratio of both signals against the pH gives a pKa of 6.45, which is appropriate for most of the measurement purposes. Furthermore, the influence of temperature, ionic strength and oxidizing substances on the performance of the fluorophores inside the dextran nanoparticles is examined. These results are compared to the dissolved dyes in order to evaluate if the dextran matrix affects the fluorescence properties of the sensor and the reference dye, and whether or not these nanosensors are suitable for pH-sensing in biological samples.

Graphical abstract: Evaluation of fluorescent polysaccharide nanoparticles for pH-sensing

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Publication details

The article was received on 07 Jan 2009, accepted on 17 Feb 2009 and first published on 12 Mar 2009


Article type: Paper
DOI: 10.1039/B900260J
Citation: Org. Biomol. Chem., 2009,7, 1884-1889
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    Evaluation of fluorescent polysaccharide nanoparticles for pH-sensing

    A. Schulz, S. Hornig, T. Liebert, E. Birckner, T. Heinze and G. J. Mohr, Org. Biomol. Chem., 2009, 7, 1884
    DOI: 10.1039/B900260J

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