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Issue 24, 2009
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Sensitive and specific enzyme immunoassays for antigenic trisaccharide from Bacillus anthracis spores

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Abstract

A straightforward synthesis of an anthrose-containing trisaccharide derived from Bacillus anthracis was achieved. Antibodies raised against this hapten provide a highly sensitive enzyme immunoassay with a detection limit of 8.5 pmol mL−1. By investigating the specificity of the antibodies obtained using different mono-, di- and trisaccharide synthetic analogues, we demonstrated that the epitope was mainly made up of the methyl group at C-5, the butamido group at C-4 and the hydroxyl at C-3 of the anthrose unit, the other parts of the trisaccharide appearing little involved in the recognition.

Graphical abstract: Sensitive and specific enzyme immunoassays for antigenic trisaccharide from Bacillus anthracis spores

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Article information


Submitted
20 Jul 2009
Accepted
16 Sep 2009
First published
19 Oct 2009

Org. Biomol. Chem., 2009,7, 5184-5199
Article type
Paper

Sensitive and specific enzyme immunoassays for antigenic trisaccharide from Bacillus anthracis spores

S. G. Y. Dhénin, V. Moreau, M. Nevers, C. Créminon and F. Djedaïni-Pilard, Org. Biomol. Chem., 2009, 7, 5184
DOI: 10.1039/B914534F

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