Jump to main content
Jump to site search

Issue 19, 2009
Previous Article Next Article

FtmOx1, a non-heme Fe(ii) and α-ketoglutarate-dependent dioxygenase, catalyses the endoperoxide formation of verruculogen in Aspergillus fumigatus

Author affiliations

Abstract

Verruculogen is a tremorgenic mycotoxin and contains an endoperoxide bond. In this study, we describe the cloning, overexpression and purification of a non-heme Fe(II) and α-ketoglutarate-dependent dioxygenase FtmOx1 from Aspergillus fumigatus, which catalyses the converstion of fumitremorgin B to verruculogen by inserting an endoperoxide bond between two prenyl moieties. Incubation with 18O2-enriched atmosphere demonstrated that both oxygen atoms of the endoperoxide bond are derived from one molecule of O2. FtmOx1 is the first endoperoxide-forming non-heme Fe(II) and α-ketoglutarate-dependent dioxygenase reported so far. A mechanism of FtmOx1-catalysed verruculogen formation is postulated and discussed.

Graphical abstract: FtmOx1, a non-heme Fe(ii) and α-ketoglutarate-dependent dioxygenase, catalyses the endoperoxide formation of verruculogen in Aspergillus fumigatus

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Apr 2009, accepted on 03 Jul 2009 and first published on 06 Aug 2009


Article type: Paper
DOI: 10.1039/B908392H
Org. Biomol. Chem., 2009,7, 4082-4087

  •   Request permissions

    FtmOx1, a non-heme Fe(II) and α-ketoglutarate-dependent dioxygenase, catalyses the endoperoxide formation of verruculogen in Aspergillus fumigatus

    N. Steffan, A. Grundmann, S. Afiyatullov, H. Ruan and S. Li, Org. Biomol. Chem., 2009, 7, 4082
    DOI: 10.1039/B908392H

Search articles by author

Spotlight

Advertisements