Enzymatic synthesis of sialylation substrates powered by a novel polyphosphate kinase (PPK3)

Abstract

Active inclusion bodies of polyphosphate kinase 3 and cytidine 5′-monophosphate kinase were combined with whole cells that co-express sialic acid aldolase and CMP-sialic acid synthetase. The biocatalytic mixture was used for the synthesis of CMP-sialic acid, which was then converted to 3′-sialyllactose by whole cells.