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Issue 23, 2009
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Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging

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Abstract

We report a method for removing unwanted contributions to fluorescence signals from dyes absorbed in polydimethylsiloxane (PDMS) using fluorescence lifetime imaging microscopy (FLIM). By analysing experimental fluorescence decays using a bi-exponential decay model, we are able to discriminate between emission originating from dye molecules in free solution and those absorbed within the PDMS substrate. Simple image processing allows the unwanted background signal to be removed and thus enables a more accurate assessment of temperature. The efficacy of the approach is demonstrated by measuring temperature changes within a droplet-based PCR device.

Graphical abstract: Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging

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Publication details

The article was received on 06 Jul 2009, accepted on 25 Aug 2009 and first published on 24 Sep 2009


Article type: Technical Note
DOI: 10.1039/B913293G
Citation: Lab Chip, 2009,9, 3437-3441
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    Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging

    T. Robinson, Y. Schaerli, R. Wootton, F. Hollfelder, C. Dunsby, G. Baldwin, M. Neil, P. French and A. deMello, Lab Chip, 2009, 9, 3437
    DOI: 10.1039/B913293G

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