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Issue 12, 2009
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Simultaneous measurement of reactions in microdroplets filled by concentration gradients

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Abstract

This work describes a technology for performing and monitoring simultaneously several reactions confined in strings of microdroplets having identical volumes but different composition, and travelling with the same speed in parallel channels of a microfluidic device. This technology, called parallel microdroplets technology (PµD), uses an inverted optical microscope and a charge-coupled device (CCD) camera to collect images and analyze them so as to report on the reactions occurring in these microdroplets. A concentration gradient of one reactant is created in the microfluidic device. In each channel, a different concentration of this reactant is mixed with a fixed amount of a second reactant. Using planar flow-focusing methodology, these mixtures are confined in microdroplets of pL size which travel in oil as continuous medium, avoiding laminar dispersion. By analyzing the images of parallel strings of microdroplets, the time courses of several reactions with different reagent compositions are investigated simultaneously. In order to design the microfluidic device that consists in a complex network of channels having well-defined geometries and restricted positions, the theoretical concept of equivalent channels (i.e. channels having identical hydraulic resistance) is exploited and developed. As a demonstration of the PµD technology, an enzyme activity assay was carried out and the steady-state kinetic constants were determined.

Graphical abstract: Simultaneous measurement of reactions in microdroplets filled by concentration gradients

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Supplementary files

Article information


Submitted
27 Nov 2008
Accepted
23 Feb 2009
First published
19 Mar 2009

Lab Chip, 2009,9, 1707-1713
Article type
Paper

Simultaneous measurement of reactions in microdroplets filled by concentration gradients

N. Damean, L. F. Olguin, F. Hollfelder, C. Abell and W. T. S. Huck, Lab Chip, 2009, 9, 1707
DOI: 10.1039/B821021G

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