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Issue 5, 2009
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Optical force sensor array in a microfluidic device based on holographic optical tweezers

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Abstract

Holographic optical tweezers (HOT) are a versatile technology, with which complex arrays and movements of optical traps can be realized to manipulate multiple microparticles in parallel and to measure the forces affecting them in the piconewton range. We report on the combination of HOT with a fluorescence microscope and a stop-flow, multi-channel microfluidic device. The integration of a high-speed camera into the setup allows for the calibration of all the traps simultaneously both using Boltzmann statistics or the power spectrum density of the particle diffusion within the optical traps. This setup permits complete spatial, chemical and visual control of the microenvironment applicable to probing chemo-mechanical properties of cellular or subcellular structures. As an example we constructed a biomimetic, quasi-two-dimensional actin network on an array of trapped polystyrene microspheres inside the microfluidic chamber. During crosslinking of the actin filaments by Mg2+ ions, we observe the build up of mechanical tension throughout the actin network. Thus, we demonstrate how our integrated HOT–microfluidics platform can be used as a reconfigurable force sensor array with piconewton resolution to investigate chemo-mechanical processes.

Graphical abstract: Optical force sensor array in a microfluidic device based on holographic optical tweezers

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Supplementary files

Article information


Submitted
07 Oct 2008
Accepted
05 Jan 2009
First published
23 Jan 2009

Lab Chip, 2009,9, 661-668
Article type
Paper

Optical force sensor array in a microfluidic device based on holographic optical tweezers

K. Uhrig, R. Kurre, C. Schmitz, J. E. Curtis, T. Haraszti, A. E.-M. Clemen and J. P. Spatz, Lab Chip, 2009, 9, 661
DOI: 10.1039/B817633G

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