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Issue 4, 2009
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A disposable, self-contained PCR chip

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Abstract

A disposable, self-contained polymerase chain reaction (PCR) chip with on-board stored, just-on-time releasable, paraffin-passivated, dry reagents is described. During both storage and sample preparation, the paraffin immobilizes and protects the stored reagents. Fluid flow through the reactor leaves the reagents undisturbed. Prior to the amplification step, the chamber is filled with target analyte suspended in water. Upon heating the PCR chamber to the DNA's denaturation temperature, the paraffin melts and moves out of the way, and the reagents are released and hydrated. To better understand the reagent release process, a scaled up model of the reactor was constructed and the paraffin migration was visualized. Experiments were carried out with a 30 µl reactor demonstrating detectable amplification (with agarose gel electrophoresis) of 10 fg (∼200 copies) of lambda DNA template. The in-reactor storage and on-time release of the PCR reagents reduce the number of needed operations and significantly simplifies the flow control that would, otherwise, be needed in lab-on-chip devices.

Graphical abstract: A disposable, self-contained PCR chip

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Supplementary files

Article information


Submitted
09 May 2008
Accepted
17 Oct 2008
First published
18 Nov 2008

Lab Chip, 2009,9, 606-612
Article type
Paper

A disposable, self-contained PCR chip

J. Kim, D. Byun, M. G. Mauk and H. H. Bau, Lab Chip, 2009, 9, 606
DOI: 10.1039/B807915C

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