Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 8, 2009
Previous Article Next Article

DNA oxidation, strand-breaks and etheno-adducts formation promoted by Cu, Zn-superoxide dismutase–H2O2 in the presence and absence of bicarbonate

Author affiliations

Abstract

Biomolecule oxidation promoted by Cu, Zn-superoxide dismutase (SOD1) has been studied because of its potential role in neurodegenerative diseases. We studied the mechanism of DNA damage promoted by the SOD1–H2O2 system. The system promoted the formation of strand breaks in plasmid DNA and the formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) in calf thymus DNA. We were also able to detect, for the first time, 1,N2-etheno-2′-deoxyguanosine (1,N2-εdGuo) in calf thymus DNA exposed to SOD1–H2O2. The addition of a copper chelator caused a decrease in the frequency of 8-oxodGuo and 1,N2-εdGuo, indicating the participation of copper ions lost from SOD1 active sites. The addition of bicarbonate increased the levels of both DNA lesions. We conclude that copper liberated from SOD1 active sites has a central role in the mechanism of DNA damage promoted by SOD1 in the presence of H2O2, and that bicarbonate can modulate the reactivity of released copper.

Graphical abstract: DNA oxidation, strand-breaks and etheno-adducts formation promoted by Cu, Zn-superoxide dismutase–H2O2 in the presence and absence of bicarbonate

Back to tab navigation

Publication details

The article was received on 31 Jul 2008, accepted on 07 Nov 2008 and first published on 15 Jan 2009


Article type: Paper
DOI: 10.1039/B813235F
Citation: Dalton Trans., 2009,0, 1450-1459

  •   Request permissions

    DNA oxidation, strand-breaks and etheno-adducts formation promoted by Cu, Zn-superoxide dismutase–H2O2 in the presence and absence of bicarbonate

    L. F. Barbosa, C. C. M. Garcia, P. Di Mascio and M. H. G. de Medeiros, Dalton Trans., 2009, 0, 1450
    DOI: 10.1039/B813235F

Search articles by author

Spotlight

Advertisements