Issue 10, 2009

Genetically encoded biosensors based on engineered fluorescent proteins

Abstract

Fluorescent proteins have revolutionized cell biology by allowing researchers to non-invasively peer into the inner workings of cells and organisms. While the most common applications of fluorescent proteins are to image expression, localization, and dynamics of protein chimeras, there is a growing interest in using fluorescent proteins to create biosensors for minimally invasive imaging of concentrations of ions and small molecules, the activity of enzymes, and changes in the conformation of proteins in living cells. This tutorial review provides an overview of the progress made in the development of fluorescent protein-based biosensors to date.

Graphical abstract: Genetically encoded biosensors based on engineered fluorescent proteins

Supplementary files

Article information

Article type
Tutorial Review
Submitted
20 Apr 2009
First published
04 Aug 2009

Chem. Soc. Rev., 2009,38, 2833-2841

Genetically encoded biosensors based on engineered fluorescent proteins

W. B. Frommer, M. W. Davidson and R. E. Campbell, Chem. Soc. Rev., 2009, 38, 2833 DOI: 10.1039/B907749A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements