Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 42, 2009
Previous Article Next Article

Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector

Author affiliations

Abstract

We reevaluate the absolute fluorescence and phosphorescence quantum yields of standard solutions by using a novel instrument developed for measuring the absolute emission quantum yields of solutions. The instrument consists of an integrating sphere equipped with a monochromatized Xe arc lamp as the light source and a multichannel spectrometer. By using a back-thinned CCD (BT-CCD) as the detector, the sensitivity for spectral detection in both the short and long wavelength regions is greatly improved compared with that of an optical detection system that uses a conventional photodetector. Using this instrument, we reevaluate the absolute fluorescence quantum yields (Φf) of some commonly used fluorescence standard solutions by taking into account the effect of reabsorption/reemission. The value of Φf for 5 × 10−3 M quinine bisulfate in 1 N H2SO4 is measured to be 0.52, which is in good agreement with the value (0.508) obtained by Melhuish by using a modified Vavilov method. In contrast, the value of Φf for 1.0 × 10−5 M quinine bisulfate in 1 N H2SO4, which is one of the most commonly used standards in quantum yield measurements based on the relative method, is measured to be 0.60. This value is significantly larger than Melhuish’s value (0.546), which was estimated by extrapolating the value of Φf for 5 × 10−3 M quinine bisulfate solution to infinite dilution using the self-quenching constant. The fluorescence quantum yield of 9,10-diphenylanthracene in cyclohexane is measured to be 0.97. This system can also be used to determine the phosphorescence quantum yields (Φp) of metal complexes that emit phosphorescence in the near-infrared region: the values of Φp for [Ru(bpy)3]2+ (bpy = 2,2′-bipyridine) are estimated to be 0.063 in water and 0.095 in acetonitrile under deaerated conditions at 298 K, while that in aerated water, which is frequently used as a luminescent reference in biological studies, is reevaluated to be 0.040.

Graphical abstract: Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector

Back to tab navigation

Supplementary files

Article information


Submitted
22 Jun 2009
Accepted
25 Aug 2009
First published
17 Sep 2009

Phys. Chem. Chem. Phys., 2009,11, 9850-9860
Article type
Paper

Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector

K. Suzuki, A. Kobayashi, S. Kaneko, K. Takehira, T. Yoshihara, H. Ishida, Y. Shiina, S. Oishi and S. Tobita, Phys. Chem. Chem. Phys., 2009, 11, 9850
DOI: 10.1039/B912178A

Social activity

Search articles by author

Spotlight

Advertisements