Issue 21, 2009

A comparative study on ganglioside micelles using electronic energy transfer, fluorescence correlation spectroscopy and light scattering techniques

Abstract

Ganglioside (GM1) micelles have been studied by means of three different techniques: fluorescence correlation spectroscopy (FCS), electronic energy transfer, as monitored by time-resolved fluorescence spectroscopy, as well as static and dynamic light scattering. The aggregation numbers obtained, 168 ± 4, remain constant over a wide range of GM1 concentrations (0.764–156 μM), are very consistent when using different donor–acceptor energy transfer pairs and have served as reference values in tests of the FCS method. It is recommended to calibrate the focal volume by using known dye concentrations. For this the rhodamine dye, 5-TAMRA, turns out to be most suitable. It is also shown that FCS provides correct values of the aggregation numbers, provided that the focal volume is calibrated by using updated values of the diffusion constant of Rhodamine 6G. These results also support recent methodological advances in FCS.

Graphical abstract: A comparative study on ganglioside micelles using electronic energy transfer, fluorescence correlation spectroscopy and light scattering techniques

Article information

Article type
Paper
Submitted
03 Dec 2008
Accepted
18 Feb 2009
First published
25 Mar 2009

Phys. Chem. Chem. Phys., 2009,11, 4335-4343

A comparative study on ganglioside micelles using electronic energy transfer, fluorescence correlation spectroscopy and light scattering techniques

R. Šachl, I. Mikhalyov, M. Hof and L. B. Å. Johansson, Phys. Chem. Chem. Phys., 2009, 11, 4335 DOI: 10.1039/B821658D

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