Issue 46, 2009
From the journal:

Chemical Communications

Enzyme-mediated protein refolding††

Jun Okada,a   Tatsuo Maruyama,*ab   Konomi Motomura,a   Kimiko Kuroki,c   Katsumi Maenaka,c   Masafumi Sakonod  and  Masahiro Goto*a  

* Corresponding authors

a Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan
E-mail: mgototcm@mbox.nc.kyushu-u.ac.jp
Fax: +81-(0)92-802-2810
Tel: +81-(0)92-802-2810

b Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-850, Japan
E-mail: tmarutcm@mbox.nc.kyushu-u.ac.jp
Fax: +81-(0)78-803-6070

c Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

d Bioengineering Laboratory, RIKEN, Hirosawa 2-1, Wako, Saitama 351-0198, Japan

Abstract

We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.

Graphical abstract: Enzyme-mediated protein refolding

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Article information

DOI
https://doi.org/10.1039/B916225A
Article type
Communication
Submitted
06 Aug 2009
Accepted
01 Oct 2009
First published
16 Oct 2009

Chem. Commun., 2009, 7197-7199

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Enzyme-mediated protein refolding

J. Okada, T. Maruyama, K. Motomura, K. Kuroki, K. Maenaka, M. Sakono and M. Goto, Chem. Commun., 2009, 7197 DOI: 10.1039/B916225A

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