Detection of        DNA using bioactive paper strips

M. Monsur Ali; Sergio D. Aguirre; Yaqin Xu; Carlos D. M. Filipe; Robert Pelton; Yingfu Li

doi:10.1039/B911559E

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Detection of DNA using bioactive paper strips†

M. Monsur Ali,^ab Sergio D. Aguirre,^ab Yaqin Xu,^c Carlos D. M. Filipe,^c Robert Pelton^c and Yingfu Li*^ab

Author affiliations

* Corresponding authors

^a Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, Ontario, Canada

^b Department of Chemistry, McMaster University, 1200 Main St. W., Hamilton, Ontario, Canada
E-mail: liying@mcmaster.ca
Fax: +1 905-522-9033

^c Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, Canada

Abstract

Paper strips containing DNA-conjugated microgels (MG) are used to achieve sensitive DNA detection in three steps: target DNA promoted ligation of a DNA primer to the MG-bound DNA, rolling circle amplification (RCA) between the primer and a circle DNA, and hybridization of the RCA products and a fluorescent DNA probe.

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Supplementary files

Experimental details for ligation and enzymaticdigestion. PDF (151K)

Article information

DOI: https://doi.org/10.1039/B911559E
Article type: Communication
Submitted: 12 Jun 2009
Accepted: 18 Aug 2009
First published: 17 Sep 2009

Download Citation

Chem. Commun., 2009, 6640-6642

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Detection of DNA using bioactive paper strips

M. M. Ali, S. D. Aguirre, Y. Xu, C. D. M. Filipe, R. Pelton and Y. Li, Chem. Commun., 2009, 6640 DOI: 10.1039/B911559E

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