Issue 9, 2009
From the journal:

Chemical Communications

Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)†

Derren J. Heyes,a   Anne-Marie Quinn,a   Paul M. Cullis,b   Michael Lee,b   Andrew W. Munroa  and  Nigel S. Scrutton*a  

* Corresponding authors

a Faculty of Life Sciences, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess St., Manchester, UK
E-mail: nigel.scrutton@manchester.ac.uk
Fax: +44 (0)161 3065201
Tel: +44 (0)161 3065152

b Department of Chemistry, University of Leicester, Leicester, UK

Abstract

We demonstrate that thiouredopyrene-3,6,8-trisulfonate (TUPS), a photoactivatable reagent, can rapidly inject electrons into complex redox enzymes, enabling studies of the kinetics of internal electron that are not accessible using conventional rapid mixing, stopped-flow methods.

Graphical abstract: Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B820386E
Article type
Communication
Submitted
14 Nov 2008
Accepted
15 Dec 2008
First published
12 Jan 2009

Chem. Commun., 2009, 1124-1126

Permissions

Request permissions

Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

D. J. Heyes, A. Quinn, P. M. Cullis, M. Lee, A. W. Munro and N. S. Scrutton, Chem. Commun., 2009, 1124 DOI: 10.1039/B820386E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements