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Issue 8, 2009
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Nanoparticle-induced potentiometric biosensing of NADH at copper ion-selective electrodes

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Abstract

We demonstrate the first example of using potentiometry at ion-selective electrodes (ISEs) for probing in real-time monitoring of biometallization processes. A copper ISE is used for real-time monitoring of the NADH-mediated reduction of copper in the presence of gold nanoparticle seeds. Such potentiometric detection of NADH is not susceptible to surface fouling common with analogous amperometric measurements of this co-factor. Biosensing of ethanol is illustrated in the presence of alcohol dehydrogenase and NAD+, along with potentiometric detection of the NADH product at the copper ISE. The concept can be readily expanded to the monitoring of various biometallization processes in connection to different enzymatic transformations and ISE, and used for ultrasensitive detection of bioaffinity interactions in connection to common enzyme tags.

Graphical abstract: Nanoparticle-induced potentiometric biosensing of NADH at copper ion-selective electrodes

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Publication details

The article was received on 03 Feb 2009, accepted on 22 Apr 2009 and first published on 15 May 2009


Article type: Paper
DOI: 10.1039/B902171J
Citation: Analyst, 2009,134, 1614-1617

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    Nanoparticle-induced potentiometric biosensing of NADH at copper ion-selective electrodes

    K. Y. Chumbimuni-Torres and J. Wang, Analyst, 2009, 134, 1614
    DOI: 10.1039/B902171J

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